Comparison of Antibacterial Effect of Nanosilver and Chlorhexidine Mouthwash on Streptococcus Sanguis (in vitro)

Abstract   Background and Aim: Streptococcus sanguis is known one of the initiating agents of microbial plaque formation. In addition to the physical control of the microbial plaque, the use of mouthwashes as a chemical method to kill microorganisms is effective. Although chlorhexidine is the gold standard for the elimination of oral microorganisms, the unpleasant side effects of its long-term use warrant a more appropriate alternative. The purpose of this study was to compare the antibacterial effect of nanosilver and chlorhexidine mouthwash on Streptococcus sanguis in pediatric department, faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University in 2018-19. Materials and methods: This experimental study was performed on Streptococcus sanguis (in vitro). Experiments were performed on two groups of case (nanosilver mouthwash) and control (chlorhexidine mouthwash) in three parts Initially, antimicrobial potency was measured by Disk diffusion method assay by measuring inhibitory zone diameter. Then, micro and macrodialysis methods were used to measure MIC (minimum inhibitory concentration) and finally to measure MBC (minimum bactericidal concentration) used the Blood agar dilution method. Results: Statistical analysis showed that the nanosilver mouthwash has no inhibitory zone. Also its MIC and MBC were higher than chlorhexine and this difference was statistically significant (P <0.001) Conclusion: According to the results of the in vitro study nanosilver mouthwash  has minor bacteriostatic and bactericidal effect compared to chlorhexidine.